Analysis Note

ControlPOSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines

Application

Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Western blot: 4 µg/mL

Immunohistochemistry (Acetone Fixed, Frozen)

ELISA

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.

Immunogen

N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).

Epitope: N-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1268

Physical form

Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.

Format: Purified

Specificity

Recognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.